Research Facilities
Proteomics
Overview
The Proteomics Core Facility provides expertise in biological mass spectrometry for large-scale protein identification and characterization, as well as quantitative proteomics-based workflows. The facility is equipped with high-performance mass spectrometers with electrospray ionization sources coupled to nano-flow liquid chromatographs - ideal for proteomics applications.
Services
Consultation
- Experimental design
- Labeling approaches and multiplexing
- Database searching (MaxQuant)
- Data filtering and imputation (Perseus)
Routine Protein Identification
- Immunoprecipitation experiments
- Purified protein samples
Complex Proteome Characterization
- with or without relative quantification
Post-Translational Modification Enrichment & Characterization
- Phosphoproteomics
- Ubiquitinomics
- Identification, localization, and quantification
Relative Quantitation Strategies
- Label Free (LFQ)
- SILAC
- SIDL
Primary Sample Processing Approaches
- s-trap
- FASP
- SDS-PAGE & gel digest
- in-solution
Training is provided for undergraduates, graduate students, post-doctoral scholars (sample processing and database searching).
Major Equipment and Instrumentation
- Hybrid Quadrupole-Orbitrap Mass Spectrometer (Q Exactive Plus, Thermo) in-line with nanoUHPLC (Ultimate 3000, Thermo)
- Offline HPLC with Fraction Collection (Ultimate 3000, Thermo)
- Ingenuity Pathway Analysis Bioinformatics Software -
Fee Schedule
Other Resources
Location and Contact Information
Proteomics Core Facility
Bio-Science Building, Suite 150
4202 E. Fowler Ave.
Tampa, FL 33620
E: Dr. Dale Chaput